Phytochemical Investigation and Spectrophotometric Studies of Piperine and Thymoquinone Compounds in Methanolic Extracts of Nigella sativa and Piper nigram spices

Abstract

<jats:p>This study was carried out on some spice samples including Nigella sativa and Piper nigram. Phytochemical screening was applied to the methanolic extraction of both the studied plants. The results showed that methanolic extract of Nigella sativa and Piper nigram revealed the presence of various chemical constituents, such as Alkaloide, Carbohydrates, Glycosides, Tannins, Flavones, Saponins. Piperine is characterizing compound present in fruits of piper nigram used as a bioavailability enhancer. Some spectrophotometric methods as (U.V) and I.R were applied to identify the major chemical compounds of the studied extracts. The results recorded that the Piperine and Thymoquinone compounds were found in the studied plant extracts.
Keywords: Piper Nigram, Nigella sativa, Piperine Thymoquinone, Spectrophotometric</jats:p>